
Minibinder Expression Kit
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Juice™ minibinder cell-free protein expression system is the only kit on the market specifically optimized for minibinder expression.
The mix can produce ~1 mg of purified minibinder constructs per milliliter of reaction in under 16 hours starting from optimized linear templates. It has been designed for T7-based protein expression and can generate proteins from standard inducible expression plasmids (e.g. pET15 and pET28) in the presence of 0.5-1mM IPTG.
An important advantage of the Juice system is that it does not contain polyethylene glycol (PEG). PEG, which is often present in other commercially available cell-free systems, is a hydrophobic agent that can result in ion suppression at the ion source of a mass spectrometer and appears as repeating elements of 44 Da in the mass spectrum (Scheerlinck et al, 2015).
Bulk cell-free is currently available in 1 mL aliquots (i.e. 334 μL of lysate per tube). If you require custom aliquots, please contact us.
Further resources:
Minibinder Cell-Free Reagents Manual
Store at -80°C.
Catalog #: CF0001.B
Publications:
A Rapid and Modular Nanobody Assay for Plug-and-Play Antigen Detection