Protein Expression Services
Protein expression services
We offer E. coli-based protein expression services both in cell-free and cell-based platforms. Our current cell-free system is ideal for rapid synthesis of proteins that can be expressed in the E. coli cytosol and is capable of making high-microgram to low-milligram quantities of purified proteins. At this time, we only express and purify poly-histidine tagged proteins.
"Liberum provided ... purified protein at a speed its competitors ... could not match. Each of these proteins Liberum shared were a therapeutic candidate & helped ... generate a data package 2 months faster than we could have otherwise. These savings compound and we will continue to leverage this service in the future to save ~6 months of a FTE's time over the next 18 months."
Services
|
Service Type |
Common Target Yield |
Timeline (Includes DNA Synthesis) |
Expected Purity |
Commercial Pricing (Includes DNA Synthesis) |
|
Turbo™ |
5-50 μg + |
~2 weeks (up to 1500 residues) |
70-90% + |
Sample protein |
|
Cell-Based Expression [3 mL] (Auto-Induction Media) |
IPTG-Inducible + SDS-PAGE of soluble and insoluble fractions |
~4 weeks |
IPTG-Inducible + SDS-PAGE of soluble and insoluble fractions |
Sample protein Add-ons: Turbo service: $99.99 (DNA synthesis included) Additional Strain: $99.99 (default is BL21 DE3) |
The system is designed for expression of cytosolic bacterial proteins. Membrane proteins and those requiring post-translational modifications such as disulfide bonds are currently not supported. If your protein has any custom requirements for optimal expression (e.g. co-factors, etc.), you are responsible for communicating such information to us at the time of ordering; timelines and pricing may be affected depending on the complexity of such requirements. Please note that some in silico designs or multiple modifications may result in insoluble proteins. The timelines are only rough estimates and may vary depending on the construct and any DNA synthesis delays.
Deliverables
- Purified proteins for each reaction (default would be in imidazole buffer: 250 mM NaCl, 250 mM imidazole, 5% glycerol, 50 mM HEPES [pH adjusted based on protein pI])
- BCA yield estimates for each target (BSA is used as standard unless you provide another standard)
- SDS-PAGE gel of purified construct
Added Services
Buffer Exchange:
- Crude (14kDa+): $29.99 USD/sample [standard buffer is 250 mM NaCl, 5% glycerol, 50 mM HEPES (pH will vary depending on your protein's pI); desalting will be done at your own risk since it has the potential to crash out the purified protein out of solution]
Shipping Fees
Secure:
Dry ice shipment via DHL
- US and Canada: $149.99 USD
- Europe: $249.99 USD (Ships on Mondays. Customer is responsible for customs clearance.)
While we make every effort to ensure a smooth delivery and also provide plenty of dry ice, we do not take responsibility for any shipping or customs delays when Standard shipping is used.
Ultra Secure:
Dry ice shipping via a specialized partner. Includes temperature monitoring throughout.
- US and Canada: $1499 USD
- Europe: $2499 USD
We will take on the risk of any shipment or customs delays when Secure shipping option is used.
How to Order
To get started, please e-mail aidan@liberumbio.com.
Examples
