Protein Expression Services

Protein expression services

We offer E. coli-based protein expression services both in cell-free and cell-based platforms. Our current cell-free system is ideal for rapid synthesis of proteins that can be expressed in the E. coli cytosol and is capable of making high-microgram to low-milligram quantities of purified proteins. At this time, we only express and purify poly-histidine tagged proteins.

"Liberum provided ... purified protein at a speed its competitors ... could not match. Each of these proteins Liberum shared were a therapeutic candidate & helped ... generate a data package 2 months faster than we could have otherwise. These savings compound and we will continue to leverage this service in the future to save ~6 months of a FTE's time over the next 18 months."

Services

Protein Expression

Service Type	Common Target Yield	Timeline (Includes DNA Synthesis)	Expected Purity	Commercial Pricing per target
Turbo™ E. coli Cell-Free (up to 1500 residues)	5-50 μg +	~2 weeks	70-90% +	Sample protein (one-time only): $149.99 + $0.30/residue Plate of 96: $99.99 + $0.30/residue 5 Plates of 96: $79.99 + $0.30/residue
VHH_His Cell-Free	5-50 μg +	~2 weeks	70-90% +	Sample protein (one-time only): $199.99 Plate of 96: $119.99

The system is designed for expression of cytosolic bacterial proteins. Membrane proteins and glycosylation are currently not available. Disulfide bond (except for VHHs) services are in beta testing stage. If your protein has any custom requirements for optimal expression (e.g. disulfide bonds, co-factors, etc.), you are responsible for communicating such information to us at the time of ordering; timelines and pricing may be affected depending on the complexity of such requirements. Please note that some in silico designs or multiple modifications may result in insoluble proteins. The timelines are only rough estimates and may vary depending on the construct and any DNA synthesis delays.

Deliverables

Purified proteins for each reaction (default would be in imidazole buffer: 250 mM NaCl, 250 mM imidazole, 5% glycerol, 50 mM HEPES [pH adjusted based on protein pI])
BCA yield estimates for each target (BSA is used as standard unless you provide another standard)
SDS-PAGE gel of purified constructs

Added Services

Buffer Exchange:

Crude (14kDa+): $29.99 USD/sample [standard buffer is 250 mM NaCl, 5% glycerol, 50 mM HEPES (pH will vary depending on your protein's pI); desalting will be done at your own risk since it has the potential to crash out the purified protein out of solution]

Shipping Fees

Secure:

Dry ice shipment via DHL

US and Canada: $149.99 USD
Europe: $249.99 USD (Ships on Mondays. Customer is responsible for customs clearance.)

While we make every effort to ensure a smooth delivery and also provide plenty of dry ice, we do not take responsibility for any shipping or customs delays when Standard shipping is used.

Ultra Secure:

Dry ice shipping via a specialized partner. Includes temperature monitoring throughout.