Protein Expression Services

Protein expression services

We offer E. coli-based protein expression services both in cell-free and cell-based platforms. Our current cell-free system is ideal for rapid synthesis of proteins that can be expressed in the E. coli cytosol and is capable of making high-microgram to low-milligram quantities of purified proteins. At this time, we only express and purify poly-histidine tagged proteins.

"Liberum provided milligrams of purified protein at a speed its competitors ... could not match.  Each of these proteins Liberum shared were a therapeutic candidate & helped ... generate a data package 2 months faster than we could have otherwise. These savings compound and we will continue to leverage this service in the future to save ~6 months of a FTE's time over the next 18 months."

 

Services

Service Type

Target Yield

Timeline (including DNA synthesis)

Expected Purity

Price (including cost of DNA synthesis)

Turbo™ (up to 400 residues only)

10-100 μg +

~2 weeks

70-90% +

$99.99 + $0.30/residue

Standard Cell-Free

100 μg - 1 mg +

~4 weeks

75-95% +

$299.99 + $0.30/residue

Boost™ (Double the yield!)

200 μg - 2 mg +

~4 weeks

75-95% +

$399.99 + $0.30/residue

Cell-Based Expression (2 x 4 mL runs for induction optimization followed by a 1 L run)

1-50 mg +

~8 weeks

75-95% +

$1499 + $0.30/residue

Secure shipping will be free for orders of 10 or more proteins.

*The system is designed for expression of cytosolic bacterial proteins. Membrane proteins and those requiring post-translational modifications such as disulfide bonds are currently not supported. If your protein has any custom requirements for optimal expression (e.g. co-factors, etc.), you are responsible for communicating such information to us at the time of ordering; timelines and pricing may be affected depending on the complexity of such requirements. Please note that some in silico designs or multiple modifications may result in insoluble proteins. The timelines are only rough estimates and may vary depending on the construct.

 

Deliverables

  1. Purified proteins for each reaction (default would be in imidazole buffer: 250 mM NaCl, 250 mM imidazole, 5% glycerol, 50 mM HEPES [pH adjusted based on protein pI])
  2. BCA yield estimates for each target (BSA is used as standard unless you provide another standard)
  3. SDS-PAGE gel of purified construct
  4. Purity estimates using densitometric analysis

 

Added Services

Buffer Exchange:

  • Crude (14kDa+): $29.99 USD/sample [standard buffer is 250 mM NaCl, 5% glycerol, 50 mM HEPES (pH will vary depending on your protein's pI); desalting will be done at your own risk since it has the potential to crash out the purified protein out of solution]

 

Shipping Fees

Secure:

Dry ice shipment via DHL

  • US and Canada: $149.99 USD
  • Europe: $249.99 USD (Ships on Mondays. Customer is responsible for customs clearance.)

While we make every effort to ensure a smooth delivery and also provide plenty of dry ice, we do not take responsibility for any shipping or customs delays when Standard shipping is used.

Ultra Secure:

Dry ice shipping via a specialized partner. Includes temperature monitoring throughout.

  • US and Canada: $1499 USD
  • Europe: $2499 USD

We will take on the risk of any shipment or customs delays when Secure shipping option is used.

 

How to Order

To get started, please e-mail aidan@liberumbio.com. 

 

Examples