Protein Expression Services

Introduction

We now offer protein expression services using our cell-free reagents and cartridges. Our current cell-free system is ideal for rapid synthesis and purification of proteins that can be expressed in the E. coli cytosol. The system is capable of making high-microgram to low-milligram quantities of purified proteins. At this time, we will only express and purify poly-histidine tagged proteins.

Individual Proteins

	Construct size	Successful* (Including cost of DNA)	Unsuccessful	Shipping
Pricing	0.3 - 1 kb	$699	Cost of DNA [This is ~$250 for a 1 kb construct. The DNA can be shipped to you for a nominal fee if you would like to try a different method.]	Free to US and Canada
	1 - 2 kb	$799
	2 - 3 kb	$899

	Expected	*Guaranteed
Yield	700 μg +	100 μg
Purity	90% +	75%

Deliverables:

Purified proteins for each reaction (default would be in imidazole buffer)
NanoDropTM yield estimates for each target
SDS-PAGE gel of purified construct
Purity estimates using densitometric analysis

Library Generation

This service allows our customers to rapidly screen libraries of protein variants. In order to minimize costs to our clients, projects will be done in three phases. As yields will vary depending on the specific target constructs, we do not guarantee specific yields for libearies; instead, we will work with you through a phased approach that minimizes your total costs.

Phase	I	II	III
Description	Small-scale testing of wild-type or representative protein	Scaled up cell-free expression of wild-type or representative protein	Scaled up cell-free expression of variants
Our Fees	Free [Phase II will automatically begin if phase I is successful]	$200	$325 for each 1 mL reaction (includes expression, purification and analysis)
Incidental Fees	Cost of DNA template generation and prep to appropriate scale (including shipping to us)	Cost of DNA template generation and prep to appropriate scale (including shipping to us)	Cost of DNA template generation and prep to appropriate scale (including shipping to us) Delivery fees
Steps	1) Identify sequence of wild type/representative target 2) Determine tagging strategy 3) Sequence optimization 4) Ordering the DNA cloned into our cell-free vector 5) Small-scale expression in cell-free	Scale up to milliliter scale and purification	1) Ordering the DNA library cloned into our cell-free vector 2) Small-scale expression in cell-free 3) Scale up to milliliter scale
Deliverables	SDS-PAGE gels from step 5	SDS-PAGE gel of purified construct NanoDrop^TM yield estimate Purity estimate using densitometric analysis	Purified proteins for each reaction (default would be in imidazole buffer) NanoDrop^TM yield estimates for each target Purity estimates using densitometric analysis

To get started, please e-mail aidan@liberumbio.com. For a demo of our system's capabilities, visit our blog post here.