Protein Expression Services
Guaranteed bacterial protein expression services
We now offer protein expression services using our bacterial cell-free reagents and our proprietary reaction scale-up technology. Our current cell-free system is ideal for rapid synthesis and purification of proteins that can be expressed in the E. coli cytosol. The system is capable of making high-microgram to low-milligram quantities of purified proteins. At this time, we will only express and purify poly-histidine tagged proteins.
"Liberum provided milligrams of purified protein at a speed its competitors ... could not match. Each of these proteins Liberum shared were a therapeutic candidate & helped ... generate a data package 2 months faster than we could have otherwise. These savings compound and we will continue to leverage this service in the future to save ~6 months of a FTE's time over the next 18 months."
Pricing
$350 + $0.30 USD per residue for each construct (includes cost of DNA synthesis)
Shipping Fees
Standard:
Dry ice shipment via DHL
- US and Canada: $149.99 USD
- Europe: $249.99 USD (Ships on Mondays. Customer is responsible for customs clearance.)
While we make every effort to ensure a smooth delivery and also provide plenty of dry ice, we do not take responsibility for any shipping or customs delays when Standard shipping is used.
Secure:
Dry ice shipping via a specialized partner. Includes temperature monitoring throughout.
- US and Canada: $1500 USD
- Europe: $2500 USD
We will take on the risk of any shipment or customs delays when Secure shipping option is used.
Deliverables
- Purified proteins for each reaction (default would be in imidazole buffer: 250 mM NaCl, 250 mM imidazole, 5% glycerol, 50 mM HEPES [pH adjusted based on protein pI])
- BCA yield estimates for each target (BSA is used as standard unless you provide another standard)
- SDS-PAGE gel of purified construct
- Purity estimates using densitometric analysis
Expected | *Guaranteed | |
Yield | 100-1000 μg + | 100 μg |
Purity | 90% + | 75% |
*Membrane proteins and those requiring post-translational modifications such as disulfide bonds are currently not supported and do not fall under our guarantee. If your protein requires a co-factor for expression, timelines and pricing may be affected. The guarantee only applies to natural proteins and single amino acid mutations made to them - we do not guarantee solubility of in silico designs.
Added Services
Buffer Exchange:
- Crude (14kDa+): $29.99 USD/sample [standard buffer is 250 mM NaCl, 5% glycerol, 50 mM HEPES (pH will vary depending on your protein's pI); desalting will be done at your own risk since it has the potential to crash out the purified protein out of solution]
Timeline
Constructs will be synthesized within a week of receiving the synthesized DNA template (total time from digital sequence to purified product is ~3-4 weeks).
How to Order
To get started, please e-mail aidan@liberumbio.com.
Examples