Protein Expression Services
Introduction
We now offer protein expression services using our cell-free reagents and cartridges. Our current cell-free system is ideal for rapid synthesis and purification of proteins that can be expressed in the E. coli cytosol. The system is capable of making high-microgram to low-milligram quantities of purified proteins. At this time, we will only express and purify poly-histidine tagged proteins.
Individual Proteins
Construct size |
Successful* (Including cost of DNA) |
Unsuccessful | Shipping | |
Pricing |
0.3 - 1 kb | $699 |
Cost of DNA [This is ~$250 for a 1 kb construct. The DNA can be shipped to you for a nominal fee if you would like to try a different method.] |
Free to US and Canada |
1 - 2 kb | $799 | |||
2 - 3 kb | $899 |
Expected | *Guaranteed | |
Yield | 700 μg + | 100 μg |
Purity | 90% + | 75% |
Deliverables:
- Purified proteins for each reaction (default would be in imidazole buffer)
- NanoDropTM yield estimates for each target
- SDS-PAGE gel of purified construct
- Purity estimates using densitometric analysis
Library Generation
This service allows our customers to rapidly screen libraries of protein variants. In order to minimize costs to our clients, projects will be done in three phases. As yields will vary depending on the specific target constructs, we do not guarantee specific yields for libearies; instead, we will work with you through a phased approach that minimizes your total costs.
Phase |
I |
II |
III |
Description |
Small-scale testing of wild-type or representative protein |
Scaled up cell-free expression of wild-type or representative protein |
Scaled up cell-free expression of variants |
Our Fees |
Free [Phase II will automatically begin if phase I is successful] |
$200 |
$325 for each 1 mL reaction (includes expression, purification and analysis) |
Incidental Fees |
Cost of DNA template generation and prep to appropriate scale (including shipping to us) |
Cost of DNA template generation and prep to appropriate scale (including shipping to us) |
Cost of DNA template generation and prep to appropriate scale (including shipping to us) Delivery fees |
Steps |
1) Identify sequence of wild type/representative target 2) Determine tagging strategy 3) Sequence optimization 4) Ordering the DNA cloned into our cell-free vector 5) Small-scale expression in cell-free |
Scale up to milliliter scale and purification |
1) Ordering the DNA library cloned into our cell-free vector 2) Small-scale expression in cell-free 3) Scale up to milliliter scale |
Deliverables |
SDS-PAGE gels from step 5 |
SDS-PAGE gel of purified construct NanoDropTM yield estimate Purity estimate using densitometric analysis |
Purified proteins for each reaction (default would be in imidazole buffer) NanoDropTM yield estimates for each target Purity estimates using densitometric analysis |
To get started, please e-mail aidan@liberumbio.com. For a demo of our system's capabilities, visit our blog post here.