Publications

Cell-Free Systems Used in Real Research

Peer-reviewed research using our cell-free technologies, proteins produced with our systems, and work involving Liberum researchers.

Cell-free expression is most useful when the resulting protein can answer a real biological question. Our systems have been used in applications ranging from functional signalling studies and nanobody diagnostics to distributed biomanufacturing.

Selected Publications

GlycoRNA complexed with heparan sulfate regulates VEGF-A signalling

Nature · 2026

A study investigating the role of glycoRNA and heparan sulfate in VEGF-A signalling.

How our technology was used: The study's methods identify cell-free E. coli protein production by Liberum Biotech for the VEGF-A165-HS wild-type and R/K proteoforms used in functional signalling experiments.

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International multisite implementation of distributed cell-free protein biomanufacturing to advance health and research equity

Science Advances · 2026

A ten-site demonstration of distributed cell-free biomanufacturing for research, diagnostics, vaccines and other bioproducts.

How our technology was used: The study used Liberum Juice alongside other cell-free systems and includes Liberum researchers among the authors.

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A Rapid and Modular Nanobody Assay for Plug-and-Play Antigen Detection

ACS Synthetic Biology · 2025

RAMONA is a modular nanobody-based antigen detection platform developed for rapid adaptation and point-of-need use.

How our technology was used: Nanobody constructs were expressed directly from linear DNA using Liberum Juice cell-free lysate and a Liberum cell-free optimized expression backbone.

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Automated Cell-free Protein Synthesis for Distributed Biomanufacturing

medRxiv · 2025 · Preprint

MANGO is an automated benchtop platform for cell-free protein synthesis and purification at the point of use.

Liberum connection: Liberum researchers are coauthors on the work, which demonstrates automated production of nanobodies, cloning enzymes and diagnostic enzymes.

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Independent Coverage

CEPI

2024

Transatlantic scientists to transition traditional vaccine development onto rapid-response platform for faster outbreak control

CEPI highlighted a £2.16 million proof-of-concept program led by CPI to develop the MANGO platform for automated, cell-free production of virus-like particle vaccines.

CEPI identifies Liberum Biotech as a consortium partner alongside CPI, Imperial College London, the University of Leeds, the University of Toronto, the University of Waterloo and Fiocruz.

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Nature

2024

Cell-free protein synthesis moves toward on-demand biomanufacturing

Nature highlighted emerging approaches to producing proteins outside living cells and referenced Liberum among companies developing cell-free protein synthesis technologies.

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IndieBio

2020

The Future of Drug Manufacturing: Just What The Doctor Ordered

IndieBio featured Liberum's cell-free protein production technology in its discussion of flexible and on-demand biologics manufacturing.

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BetaKit

2023

Liberum raises $1.8 million for protein synthesis as a service

BetaKit covered Liberum's seed financing and its work to accelerate recombinant protein production using cell-free technology.

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TechCrunch

2020

IndieBio Demo Day: Liberum's protein printer

TechCrunch profiled Liberum during IndieBio's Demo Day, highlighting its approach to rapid recombinant protein production.

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Put Cell-Free Expression to Work

Ready-to-use bacterial, yeast and mammalian cell-free systems make it possible to move from DNA to protein without maintaining living expression cultures.

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